Human PDHB activation kit by CRISPRa

CAT#: GA103459

PDHB CRISPRa kit - CRISPR gene activation of human pyruvate dehydrogenase E1 beta subunit

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PDHB
• Locus ID: 5162
• Kit Components:

  GA103459G1, PDHB gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103459G2, PDHB gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103459G3, PDHB gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000925, NM_001173468, NM_001315536, NR_033384
• Synonyms: PDHBD; PDHE1-B; PHE1B
• Summary: 'The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012]'