Human PFKFB3 activation kit by CRISPRa

CAT#: GA103499

PFKFB3 CRISPRa kit - CRISPR gene activation of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PFKFB3
• Locus ID: 5209
• Kit Components:

  GA103499G1, PFKFB3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103499G2, PFKFB3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103499G3, PFKFB3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001145443, NM_001282630, NM_001314063, NM_001323016, NM_001323017, NM_004566, NR_136554, NM_001363545
• Synonyms: iPFK-2; IPFK2; PFK2
• Summary: 'The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]'