Human Phospholipase C gamma 1 (PLCG1) activation kit by CRISPRa

CAT#: GA103595

PLCG1 CRISPRa kit - CRISPR gene activation of human phospholipase C gamma 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PLCG1
• Locus ID: 5335
• Kit Components:

  GA103595G1, Phospholipase C gamma 1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103595G2, Phospholipase C gamma 1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103595G3, Phospholipase C gamma 1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002660, NM_182811
• Synonyms: NCKAP3; PLC-II; PLC1; PLC148; PLCgamma1
• Summary: 'The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'