Human PLOD1 activation kit by CRISPRa

CAT#: GA103610

PLOD1 CRISPRa kit - CRISPR gene activation of human procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PLOD1
• Locus ID: 5351
• Kit Components:

  GA103610G1, PLOD1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103610G2, PLOD1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103610G3, PLOD1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000302, NM_001316320
• Synonyms: EDS6; LH; LH1; LLH; PLOD
• Summary: 'Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]'