Human DNA polymerase eta (POLH) activation kit by CRISPRa

CAT#: GA103652

POLH CRISPRa kit - CRISPR gene activation of human DNA polymerase eta

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: POLH
• Locus ID: 5429
• Kit Components:

  GA103652G1, DNA polymerase eta gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103652G2, DNA polymerase eta gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103652G3, DNA polymerase eta gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001291969, NM_001291970, NM_006502
• Synonyms: RAD30; RAD30A; XP-V; XPV
• Summary: 'This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]'