Human AMPK alpha 2 (PRKAA2) activation kit by CRISPRa

CAT#: GA103755

PRKAA2 CRISPRa kit - CRISPR gene activation of human protein kinase AMP-activated catalytic subunit alpha 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PRKAA2
• Locus ID: 5563
• Kit Components:

  GA103755G1, AMPK alpha 2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103755G2, AMPK alpha 2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103755G3, AMPK alpha 2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_006252
• Synonyms: AMPK; AMPK2; AMPKa2; PRKAA
• Summary: 'The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]'