Human PKA R2 (PRKAR2A) activation kit by CRISPRa

CAT#: GA103766

PRKAR2A CRISPRa kit - CRISPR gene activation of human protein kinase cAMP-dependent type II regulatory subunit alpha

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PRKAR2A
• Locus ID: 5576
• Kit Components:

  GA103766G1, PKA R2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103766G2, PKA R2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103766G3, PKA R2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_004157, NM_001321982, NM_001321983, NM_001321989
• Synonyms: PKR2; PRKAR2
• Summary: 'cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]'