Human PKN1 activation kit by CRISPRa

CAT#: GA103775

PKN1 CRISPRa kit - CRISPR gene activation of human protein kinase N1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PKN1
• Locus ID: 5585
• Kit Components:

  GA103775G1, PKN1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103775G2, PKN1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103775G3, PKN1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002741, NM_213560
• Synonyms: DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1; PRKCL1
• Summary: 'The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'