Human RAF1 activation kit by CRISPRa

CAT#: GA104007

RAF1 CRISPRa kit - CRISPR gene activation of human Raf-1 proto-oncogene, serine/threonine kinase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: RAF1
• Locus ID: 5894
• Kit Components:

  GA104007G1, RAF1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104007G2, RAF1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104007G3, RAF1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002880, NM_001354689, NM_001354690, NM_001354691, NM_001354692, NM_001354693, NM_001354694, NM_001354695, NR_148940, NR_148941, NR_148942
• Synonyms: c-Raf; CMD1NN; CRAF; NS5; Raf-1
• Summary: 'This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]'