Human SAFB activation kit by CRISPRa

CAT#: GA104269

SAFB CRISPRa kit - CRISPR gene activation of human scaffold attachment factor B

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SAFB
• Locus ID: 6294
• Kit Components:

  GA104269G1, SAFB gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104269G2, SAFB gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104269G3, SAFB gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001201338, NM_001201339, NM_001201340, NM_001320571, NM_001320572, NM_002967, NR_037699
• Synonyms: HAP; HET; SAB-B1; SAF-B; SAF-B1; SAFB1
• Summary: 'This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]'