Human TGIF (TGIF1) activation kit by CRISPRa

CAT#: GA104857

TGIF1 CRISPRa kit - CRISPR gene activation of human TGFB induced factor homeobox 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TGIF1
• Locus ID: 7050
• Kit Components:

  GA104857G1, TGIF gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104857G2, TGIF gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104857G3, TGIF gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001278682, NM_001278684, NM_001278686, NM_003244, NM_170695, NM_173207, NM_173208, NM_173209, NM_173210, NM_173211, NM_174886
• Synonyms: HPE4; TGIF
• Summary: 'The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described. [provided by RefSeq, Jul 2013]'