Human Topoisomerase III alpha (TOP3A) activation kit by CRISPRa

CAT#: GA104941

TOP3A CRISPRa kit - CRISPR gene activation of human DNA topoisomerase III alpha

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TOP3A
• Locus ID: 7156
• Kit Components:

  GA104941G1, Topoisomerase III alpha gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104941G2, Topoisomerase III alpha gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104941G3, Topoisomerase III alpha gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001320759, NM_004618
• Synonyms: TOP3; ZGRF7
• Summary: 'This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]'