Human Vitamin D Receptor (VDR) activation kit by CRISPRa

CAT#: GA105114

VDR CRISPRa kit - CRISPR gene activation of human vitamin D receptor

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: VDR
• Locus ID: 7421
• Kit Components:

  GA105114G1, Vitamin D Receptor gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA105114G2, Vitamin D Receptor gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA105114G3, Vitamin D Receptor gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000376, NM_001017535, NM_001017536, NM_001364085
• Synonyms: NR1I1; PPP1R163
• Summary: 'This gene encodes vitamin D3 receptor, which is a member of the nuclear hormone receptor superfamily of ligand-inducible transcription factors. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. Downstream targets of vitamin D3 receptor are principally involved in mineral metabolism, though this receptor regulates a variety of other metabolic pathways, such as those involved in immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jun 2018]'