Human PRAK (MAPKAPK5) activation kit by CRISPRa

CAT#: GA105664

MAPKAPK5 CRISPRa kit - CRISPR gene activation of human MAPK activated protein kinase 5

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: MAPKAPK5
• Locus ID: 8550
• Kit Components:

  GA105664G1, MAPKAPK5 gRNA vector 1 in pCas-Guide-CRISPRa

  GA105664G2, MAPKAPK5 gRNA vector 2 in pCas-Guide-CRISPRa

  GA105664G3, MAPKAPK5 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_003668, NM_139078
• Synonyms: MAPKAP-K5; MK-5; MK5; PRAK
• Summary: The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012]