Human DPH5 activation kit by CRISPRa

CAT#: GA109901

DPH5 CRISPRa kit - CRISPR gene activation of human diphthamide biosynthesis 5

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DPH5
• Locus ID: 51611
• Kit Components:

  GA109901G1, DPH5 gRNA vector 1 in pCas-Guide-CRISPRa

  GA109901G2, DPH5 gRNA vector 2 in pCas-Guide-CRISPRa

  GA109901G3, DPH5 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001077394, NM_001077395, NM_015958
• Synonyms: AD-018; CGI-30; HSPC143; NPD015
• Summary: This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]