Human Slingshot homolog 1 (SSH1) activation kit by CRISPRa

CAT#: GA110097

SSH1 CRISPRa kit - CRISPR gene activation of human slingshot protein phosphatase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SSH1
• Locus ID: 54434
• Kit Components:

  GA110097G1, SSH1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA110097G2, SSH1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA110097G3, SSH1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001161330, NM_001161331, NM_018984
• Synonyms: SSH1L
• Summary: The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]