Human RNF43 activation kit by CRISPRa

CAT#: GA110350

RNF43 CRISPRa kit - CRISPR gene activation of human ring finger protein 43

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: RNF43
• Locus ID: 54894
• Kit Components:

  GA110350G1, RNF43 gRNA vector 1 in pCas-Guide-CRISPRa

  GA110350G2, RNF43 gRNA vector 2 in pCas-Guide-CRISPRa

  GA110350G3, RNF43 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001305544, NM_001305545, NM_017763
• Synonyms: RNF124; SSPCS; URCC
• Summary: The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]