Human Protor 1 (PRR5) activation kit by CRISPRa

CAT#: GA110825

PRR5 CRISPRa kit - CRISPR gene activation of human proline rich 5

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PRR5
• Locus ID: 55615
• Kit Components:

  GA110825G1, PRR5 gRNA vector 1 in pCas-Guide-CRISPRa

  GA110825G2, PRR5 gRNA vector 2 in pCas-Guide-CRISPRa

  GA110825G3, PRR5 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001017528, NM_001017529, NM_001017530, NM_001198721, NM_015366, NM_181333
• Synonyms: FLJ20185k; PP610; PROTOR-1; PROTOR1
• Summary: This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010]