Human TYW1B activation kit by CRISPRa

CAT#: GA118497

TYW1B CRISPRa kit - CRISPR gene activation of human tRNA-yW synthesizing protein 1 homolog B

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TYW1B
• Locus ID: 441250
• Kit Components:

  GA118497G1, TYW1B gRNA vector 1 in pCas-Guide-CRISPRa

  GA118497G2, TYW1B gRNA vector 2 in pCas-Guide-CRISPRa

  GA118497G3, TYW1B gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001145440, NM_001145441, NR_027031
• Synonyms: LINC00069; NCRNA00069; RSAFD2
• Summary: Wybutosine is a hypermodified guanosine found in phenylalanine tRNA. Wybutosine functions to stabilize codon-anticodon interactions during ribosome decoding and therefore supports the maintenance of the reading frame. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. The human genome contains two closely related genes that putatively function in wybutosine synthesis. The open reading frame of this locus is disrupted in some individuals. Thus, this locus appears to be an evolving pseudogene, but may still be functional in some members of the population. [provided by RefSeq, Apr 2014]