Mouse Akt2 activation kit by CRISPRa

CAT#: GA200151

Akt2 CRISPRa kit - CRISPR gene activation of mouse thymoma viral proto-oncogene 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Akt2
• Locus ID: 11652
• Kit Components:

  GA200151G1, Akt2 gRNA vector 1 in pCas-Guide-CRISPRa

  GA200151G2, Akt2 gRNA vector 2 in pCas-Guide-CRISPRa

  GA200151G3, Akt2 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001110208, NM_001331108, NM_001331109, NM_007434
• Synonyms: 2410016A19Rik; AW554154; PKB; PKBbeta
• Summary: One of the few specific substrates of AKT2 identified so far is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during insulin-stimulated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Phosphorylates CLK2 on 'Thr-343'. [UniProtKB/Swiss-Prot Function]