Mouse Cntfr activation kit by CRISPRa

CAT#: GA200796

Cntfr CRISPRa kit - CRISPR gene activation of mouse ciliary neurotrophic factor receptor

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Cntfr
• Locus ID: 12804
• Kit Components:

  GA200796G1, Cntfr gRNA vector 1 in pCas-Guide-CRISPRa

  GA200796G2, Cntfr gRNA vector 2 in pCas-Guide-CRISPRa

  GA200796G3, Cntfr gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001136056, NM_001146080, NM_016673
• Synonyms: Cntfralpha
• Summary: This gene encodes the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor that triggers the assembly of a trimolecular complex upon binding to CNTF, and initiate a downstream signaling process. The encoded preproprotein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-linked cell surface protein. Mice lacking the encoded protein die shortly after birth and exhibit a reduction of motoneuron number at birth. The transgenic disruption of this gene specifically in the skeletal muscle followed by a peripheral nerve lesion impairs motor neuron axonal regeneration across the lesion site. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]