Mouse Col4a1 activation kit by CRISPRa

CAT#: GA200814

Col4a1 CRISPRa kit - CRISPR gene activation of mouse collagen, type IV, alpha 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Col4a1
• Locus ID: 12826
• Kit Components:

  GA200814G1, Col4a1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA200814G2, Col4a1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA200814G3, Col4a1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_009931, NR_133670
• Synonyms: Bru; Col4a-1; Raw; Svc
• Summary: This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]