Mouse Mbd3 activation kit by CRISPRa

CAT#: GA202592

Mbd3 CRISPRa kit - CRISPR gene activation of mouse methyl-CpG binding domain protein 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Mbd3
• Locus ID: 17192
• Kit Components:

  GA202592G1, Mbd3 gRNA vector 1 in pCas-Guide-CRISPRa

  GA202592G2, Mbd3 gRNA vector 2 in pCas-Guide-CRISPRa

  GA202592G3, Mbd3 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001306143, NM_013595
• Synonyms: AI181826; AU019209
• Summary: This gene encodes a member of the MBD family of nuclear proteins that contain a methyl-CpG binding domain (MBD). The encoded protein is a component of the nucleosome remodeling and histone deacetylation (NuRD) complex. Deletion of this gene causes embryonic lethality in mice. Embryonic stem cells lacking the encoded protein are severely compromised in their ability to differentiate and fail to commit to developmental lineages in the absence of leukemia inhibitory factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]