Mouse Pcsk5 activation kit by CRISPRa

CAT#: GA203128

Pcsk5 CRISPRa kit - CRISPR gene activation of mouse proprotein convertase subtilisin/kexin type 5

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Pcsk5
• Locus ID: 18552
• Kit Components:

  GA203128G1, Pcsk5 gRNA vector 1 in pCas-Guide-CRISPRa

  GA203128G2, Pcsk5 gRNA vector 2 in pCas-Guide-CRISPRa

  GA203128G3, Pcsk5 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001163144, NM_001190483
• Synonyms: b2b585Clo; b2b1549Clo; PC5; PC6; SPC6
• Summary: This gene encodes a subtilisin-like proprotein convertase that mediates posttranslational endoproteolytic processing of various proprotein substrates traversing the secretory pathway. The encoded protein is an inactive zymogen that undergoes autoproteolytic processing in the endoplasmic reticulum and the Golgi network to generate an active enzyme. Mice lacking the encoded protein die at an early embryonic stage. Conditional inactivation this gene in the epiblast but not in the extraembryonic tissue bypasses embryonic lethality but results in death at birth. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]