Mouse Pum3 activation kit by CRISPRa

CAT#: GA205532

Pum3 CRISPRa kit - CRISPR gene activation of mouse pumilio RNA-binding family member 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Pum3
• Locus ID: 52874
• Kit Components:

  GA205532G1, Pum3 gRNA vector 1 in pCas-Guide-CRISPRa

  GA205532G2, Pum3 gRNA vector 2 in pCas-Guide-CRISPRa

  GA205532G3, Pum3 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_177474, NR_131940
• Synonyms: 1110069H02Rik; AA675048; D19Bwg1357e; Kiaa0020
• Summary: This gene encodes a member of the evolutionarily conserved Pumilio and Fem-3 mRNA-binding factor family of proteins, which are characterized by tandem 36 amino acid pumilio homolog domains and which function in diverse biological processes. This protein belongs to a group of atypical Pumilio and Fem-3 mRNA-binding factor proteins, whose members are distinguished from other Pumilio and Fem-3 mRNA-binding factor proteins by a novel protein fold with 11 pumilio homolog domains and an ability to bind to DNA and single- and double-stranded RNA without sequence specificity. In mouse, lower levels of gene expression have been correlated with increased testicular germ cell tumors. A pseudogene of this gene is found on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]