Mouse Fancl activation kit by CRISPRa

CAT#: GA207094

Fancl CRISPRa kit - CRISPR gene activation of mouse Fanconi anemia, complementation group L

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Fancl
• Locus ID: 67030
• Kit Components:

  GA207094G1, Fancl gRNA vector 1 in pCas-Guide-CRISPRa

  GA207094G2, Fancl gRNA vector 2 in pCas-Guide-CRISPRa

  GA207094G3, Fancl gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001277273, NM_025923, NR_102382
• Synonyms: 2010322C19Rik; AW554273; B230118H11Rik; gcd; Phf9; Pog
• Summary: This gene encodes the complementation group L subunit of the multimeric Fanconi anemia (FA) nuclear complex composed of proteins encoded by over ten Fanconi anemia complementation (FANC) group genes. The FA complex is necessary for protection against DNA damage. This gene product, an E3 ubiquitin ligase, catalyzes and is required for the monoubiquitination of the protein encoded by the Fanconi anemia, complementation group D2 gene, a critical step in the FA pathway (PMID: 12973351, 21229326). In mouse, mutations of this E3 ubiquitin ligase gene can lead to infertility in adult males and females, and a deletion of this gene can cause embryonic lethality in some genetic backgrounds. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]