Mouse Mms19 activation kit by CRISPRa

CAT#: GA209149

Mms19 CRISPRa kit - CRISPR gene activation of mouse MMS19 cytosolic iron-sulfur assembly component

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Mms19
• Locus ID: 72199
• Kit Components:

  GA209149G1, Mms19 gRNA vector 1 in pCas-Guide-CRISPRa

  GA209149G2, Mms19 gRNA vector 2 in pCas-Guide-CRISPRa

  GA209149G3, Mms19 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_028152
• Synonyms: 2410001K24Rik; 2610042O15Rik; AI316855; C79368; C86341; Mms19l
• Summary: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER) and RNA polymerase II (POL II) transcription. As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD. Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery. [UniProtKB/Swiss-Prot Function]