Mouse B3gnt5 activation kit by CRISPRa

CAT#: GA212091

B3gnt5 CRISPRa kit - CRISPR gene activation of mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: B3gnt5
• Locus ID: 108105
• Kit Components:

  GA212091G1, B3gnt5 gRNA vector 1 in pCas-Guide-CRISPRa

  GA212091G2, B3gnt5 gRNA vector 2 in pCas-Guide-CRISPRa

  GA212091G3, B3gnt5 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001159407, NM_001159408, NM_054052
• Synonyms: beta3GnT5
• Summary: Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development. [UniProtKB/Swiss-Prot Function]