Stable Cell Line

CAT#: SL500073

Human Doublecortin-like Kinase 1 DCAMKL1 Stable Cell Line - NIH3T3

Product Images

Figure 1. Western blot analysis for NIH3T3/ DCAMKL1 using mAb against DDK (FLAG) tag (Cat# TA50011-100). Protein concentrations were measured by BCA kit (Thermo Scientific Inc.). Lane A: NIH3T3/ DCAMKL1 Lysate at Passage 1 from Master Cell Bank, 15 ug; Lane B: NIH3T3/ DCAMKL1 Lysate at Passage 6 from Master Cell Bank, 15 ug ; Lane C: Untransfected NIH3T3 cells Lysate was used as negative control, 15 ug.

Specifications

Product Data

• Gene: DCLK1
• Accn: NM_004734
• Synonyms: CL1; CLICK1; DCAMKL1; DCDC3A; DCLK
• Host Cell: NIH3T3
• Format: Cryopreserved cells, 2 x 10E6 cells/vial
• Mycoplasma: Negative by MycoAlert Kit, Lonza
• Cell Line Validation: Western blot
• Background: Doublecortin-like Kinase 1 (DCAMKL1) is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, a C-terminal serine/threonine protein kinase domain and a serine/proline-rich domain which is between the doublecortin and the protein kinase domains. Different domains reveal alterative functions. N-terminal domains bind microtubules and regulate microtubule polymerization. The C-terminal domain shows substantial homology to Ca2+/calmodulin-dependent protein kinase. However, the serine/proline-rich domain functions in mediating multiple protein-protein interactions. DCAMKL1 involves in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. DCAMKL1 expression is up-regulated by brain-derived neurotrophic factor and is associated with memory and general cognitive abilities.