Flotillin 1 (FLOT1) (NM_005803) Human Mass Spec Standard

CAT#: PH300231

FLOT1 MS Standard C13 and N15-labeled recombinant protein (NP_005794)

Product Images

Coomassie blue staining of purified FLOT1 protein (Cat# TP300231). The protein was produced from HEK293T cells transfected with FLOT1 cDNA clone (Cat# RC200231) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200231
• Predicted MW: 47.4 kDa
• Protein Sequence:
  Sequence Link

  >RC200231 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQ
  VKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLV
  NMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMA
  KAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELE
  ARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQE
  AAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVN
  HKPLRTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005794
• RefSeq Size: 1839
• RefSeq ORF: 1281
• Synonyms: flotillin 1; integral membrane component of caveolae; OTTHUMP00000029208
• Locus ID: 10211
• UniProt ID: O75955, Q5ST80
• Cytogenetics: 6p21.33
• Summary: This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
• Protein Pathways: Insulin signaling pathway