Uridine Phosphorylase 1 (UPP1) (NM_003364) Human Mass Spec Standard

CAT#: PH300406

UPP1 MS Standard C13 and N15-labeled recombinant protein (NP_003355)

Product Images

Coomassie blue staining of purified UPP1 protein (Cat# TP300406). The protein was produced from HEK293T cells transfected with UPP1 cDNA clone (Cat# RC200406) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200406
• Predicted MW: 33.9 kDa
• Protein Sequence:
  Sequence Link

  >RC200406 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATGANAEKAESHNDCPVRLLNPNIAKMKEDILYHFNLTTSRHNFPALFGDVKFVCVGGSPSRMKAFIR
  CVGAELGLDCPGRDYPNICAGTDRYAMYKVGPVLSVSHGMGIPSISIMLHELIKLLYYARCSNVTIIRIG
  TSGGIGLEPGTVVITEQAVDTCFKAEFEQIVLGKRVIRKTDLNKKLVQELLLCSAELSEFTTVVGNTMCT
  LDFYEGQGRLDGALCSYTEKDKQAYLEAAYAAGVRNIEMESSVFAAMCSACGLQAAVVCVTLLNRLEGDQ
  ISSPRNVLSEYQQRPQRLVSYFIKKKLSKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_003355
• RefSeq Size: 1733
• RefSeq ORF: 930
• Synonyms: UDRPASE; UP; UPASE; UPP
• Locus ID: 7378
• UniProt ID: Q16831, B4DND0
• Cytogenetics: 7p12.3
• Summary: 'This gene encodes a uridine phosphorylase, an enzyme that catalyzes the reversible phosphorylation of uridine (or 2'- deoxyuridine) to uracil and ribose-1-phosphate (or deoxyribose-1-phosphate). The encoded enzyme functions in the degradation and salvage of pyrimidine ribonucleosides. [provided by RefSeq, Oct 2016]'
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism