ELOB (NM_007108) Human Mass Spec Standard

CAT#: PH301393

TCEB2 MS Standard C13 and N15-labeled recombinant protein (NP_009039)

Product Images

Coomassie blue staining of purified ELOB protein (Cat# TP301393). The protein was produced from HEK293T cells transfected with ELOB cDNA clone (Cat# RC201393) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201393
• Predicted MW: 13.1 kDa
• Protein Sequence:
  Sequence Link

  >RC201393 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPDEQRLYKDDQLLDDGKTLGECGFTSQTARPQ
  APATVGLAFRADDTFEALCIEPFSSPPELPDVMKPQDSGSSANEQAVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_009039
• RefSeq Size: 1009
• RefSeq ORF: 354
• Synonyms: SIII; TCEB2
• Locus ID: 6923
• UniProt ID: Q15370
• Cytogenetics: 16p13.3
• Summary: 'This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13. [provided by RefSeq, Aug 2008]'
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis