NDOR1 (NM_014434) Human Mass Spec Standard

CAT#: PH304845

NDOR1 MS Standard C13 and N15-labeled recombinant protein (NP_055249)

Product Images

Coomassie blue staining of purified NDOR1 protein (Cat# TP304845). The protein was produced from HEK293T cells transfected with NDOR1 cDNA clone (Cat# RC204845) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC204845
• Predicted MW: 66.8 kDa
• Protein Sequence:
  Sequence Link

  >RC204845 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSPQLLVLFGSQTGTAQDVSERLGREARRRRLGCRVQALDSYPVVNLINEPLVIFVCATTGQGDPPDNM
  KNFWRFIFRKNLPSTALCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQLGGSALLPVCLGDDQHELGPDA
  AVDPWLRDLWDRVLGLYPPPPGLTEIPPGVPLPSKFTLLFLQEAPSTGSEGQRVAHPGSQEPPSESKPFL
  APMISNQRVTGPSHFQDVRLIEFDILGSGISFAAGDVVLIQPSNSAAHVQRFCQVLGLDPDQLFMLQPRE
  PDVSSPTRLPQPCSMRHLVSHYLDIASVPRRSFFELLACLSLHELEREKLLEFSSAQGQEELFEYCNRPR
  RTILEVLCDFPHTAAAIPPDYLLDLIPVIRPRAFSIASSLLTHPSRLQILVAVVQFQTRLKEPRRGLCSS
  WLASLDPGQGPVRVPLWVRPGSLAFPETPDTPVIMVGPGTGVAPFRAAIQERVAQGQTGNFLFFGCRWRD
  QDFYWEAEWQELEKRDCLTLIPAFSREQEQKIYVQHRLRELGSLVWELLDRQGAYFYLAGNAKSMPADVS
  EALMSIFQEEGGLCSPDAAAYLARLQQTRRFQTETWA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_055249
• RefSeq Size: 4850
• RefSeq ORF: 1791
• Synonyms: bA350O14.9; CIAE1; NR1
• Locus ID: 27158
• UniProt ID: Q9UHB4
• Cytogenetics: 9q34.3
• Summary: This gene encodes an NADPH-dependent diflavin reductase that contains both flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) binding domains. The encoded protein catalyzes the transfer of electrons from NADPH through FAD and FMN cofactors to potential redox partners. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]