VPS36 (NM_016075) Human Mass Spec Standard

CAT#: PH306006

VPS36 MS Standard C13 and N15-labeled recombinant protein (NP_057159)

Product Images

Coomassie blue staining of purified VPS36 protein (Cat# TP306006). The protein was produced from HEK293T cells transfected with VPS36 cDNA clone (Cat# RC206006) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC206006
• Predicted MW: 43.8 kDa
• Protein Sequence:
  Sequence Link

  >RC206006 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIE
  EQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSL
  QTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDIT
  EDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGME
  LLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSV
  LLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057159
• RefSeq Size: 4473
• RefSeq ORF: 1158
• Synonyms: C13orf9; CGI-145; EAP45
• Locus ID: 51028
• UniProt ID: Q86VN1
• Cytogenetics: 13q14.3
• Summary: This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013]
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis