NLK (NM_016231) Human Mass Spec Standard

CAT#: PH308723

NLK MS Standard C13 and N15-labeled recombinant protein (NP_057315)

Product Images

Coomassie blue staining of purified NLK protein (Cat# TP308723). The protein was produced from HEK293T cells transfected with NLK cDNA clone (Cat# RC208723) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC208723
• Predicted MW: 58.3 kDa
• Protein Sequence:
  Sequence Link

  >RC208723 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLCGARANAKMMAAYNGGTSAAATGHHHHHHHHLPHLPPPHLHHHHHPQHHLHPGSAAAVHPVQQHTSS
  AAAAAAAAAAAAAMLNPGQQQPYFPSPAPGQAPGPAAAAPAQVQAAAAATVKAHHHQHSHHPQQQLDIEP
  DRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPHID
  YFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKI
  CDFGLARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAELLGRRILFQAQSPIQQL
  DLITDLLGTPSLEAMRTACEGAKAHILRGPHKQPSLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKD
  ALAHPYLDEGRLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEIIHQFILEQQK
  GNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057315
• RefSeq Size: 3555
• RefSeq ORF: 1581
• Synonyms: DKFZp761G1211; FLJ21033
• Locus ID: 51701
• UniProt ID: Q9UBE8, A0A024QZ12
• Cytogenetics: 17q11.2
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Adherens junction, MAPK signaling pathway, Wnt signaling pathway