JIP1 (MAPK8IP1) (NM_005456) Human Mass Spec Standard

CAT#: PH309734

MAPK8IP1 MS Standard C13 and N15-labeled recombinant protein (NP_005447)

Product Images

Coomassie blue staining of purified MAPK8IP1 protein (Cat# TP309734). The protein was produced from HEK293T cells transfected with MAPK8IP1 cDNA clone (Cat# RC209734) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209734
• Predicted MW: 77.5 kDa
• Protein Sequence:
  Sequence Link

  >RC209734 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAERESGGLGGGAASPPAASPFLGLHIASPPNFRLTHDISLEEFEDEDLSEITDECGISLQCKDTLSLRP
  PRAGLLSAGGGGAGSRLQAEMLQMDLIDATGDTPGAEDDEEDDDEERAARRPGAGPPKAESGQEPASRGQ
  GQSQGQSQGPGSGDTYRPKRPTTLNLFPQVPRSQDTLNNNSLGKKHSWQDRVSRSSSPLKTGEQTPPHEH
  ICVSDELSPQSGPAPTTGRGTSTDSPCRRSTATQMAPPGGPPAATPGGRGHSHRDRIHYQADVRLEATEE
  IYLTPVQRPPDAAEPTSAFLPPTESRMSVSSDPDPAAYPSTAGRPHPSISEEEEGFDCMSSPERAEPPGG
  GWRGSLGEPPPPPRASLSSDTSALSYDSVKYTLVVDEHAQLELVSLRPCFGDYSDESDSATVYDNCASVS
  SPYESAIGEEYEEAPRPQPPACLSEDSTPDEPDVHFSKKFLNVFMSGRSRSSSAESFGLFSCIINGEEQE
  QTHRAIFRFVPRHEDELELEVDDPLLVELQAEDYWYEAYNMRTGARGVFPAYYAIEVTKEPEHMAALAKN
  SDWVDQFRVKFLGSVQVPYHKGNDVLCAAMQKIATTRRLTVHFNPPSSCVLEINVRGVKIGVKADDSQEA
  KGNKCSHFFQLKNISFCGYHPKNNKYFGFITKHPADNRFACHVFVSEDSTKALAESVGRAFQQFYKQFVE
  YTCPTEDIYLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005447
• RefSeq Size: 3234
• RefSeq ORF: 2133
• Synonyms: IB1; JIP-1; JIP1; PRKM8IP
• Locus ID: 9479
• UniProt ID: Q9UQF2, Q6NUQ9
• Cytogenetics: 11p11.2
• Summary: This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq, May 2011]
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway