HIBCH (NM_014362) Human Mass Spec Standard

CAT#: PH309814

HIBCH MS Standard C13 and N15-labeled recombinant protein (NP_055177)

Product Images

Coomassie blue staining of purified HIBCH protein (Cat# TP309814). The protein was produced from HEK293T cells transfected with HIBCH cDNA clone (Cat# RC209814) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209814
• Predicted MW: 43.4 kDa
• Protein Sequence:
  Sequence Link

  >RC209814 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLGKKGCTGVITLNRPKFLNALTLNMIRQIYP
  QLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIH
  GITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRA
  GIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEI
  IENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVL
  IDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_055177
• RefSeq Size: 1958
• RefSeq ORF: 1158
• Synonyms: HIBYLCOAH
• Locus ID: 26275
• UniProt ID: Q6NVY1, A0A140VJL0
• Cytogenetics: 2q32.2
• Summary: This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
• Protein Pathways: beta-Alanine metabolism, Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation