Securin (PTTG1) (NM_004219) Human Mass Spec Standard

CAT#: PH311038

PTTG1 MS Standard C13 and N15-labeled recombinant protein (NP_004210)

Product Images

Coomassie blue staining of purified PTTG1 protein (Cat# TP311038). The protein was produced from HEK293T cells transfected with PTTG1 cDNA clone (Cat# RC211038) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC211038
• Predicted MW: 22 kDa
• Protein Sequence:
  Sequence Link

  >RC211038 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTPRFGKTFDAPPALPKATRKALGTVNRA
  TEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLP
  LSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQSPSSILSTLDVELPPVCCDIDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004210
• RefSeq Size: 786
• RefSeq ORF: 606
• Synonyms: EAP1; HPTTG; PTTG; TUTR1
• Locus ID: 9232
• UniProt ID: O95997, Q6IAL9
• Cytogenetics: 5q33.3
• Summary: The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, Oocyte meiosis