Apolipoprotein L 1 (APOL1) (NM_003661) Human Mass Spec Standard

CAT#: PH311114

APOL1 MS Standard C13 and N15-labeled recombinant protein (NP_003652)

Product Images

Coomassie blue staining of purified APOL1 protein (Cat# TP311114). The protein was produced from HEK293T cells transfected with APOL1 cDNA clone (Cat# RC211114) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC211114
• Predicted MW: 43.97 kDa
• Protein Sequence:
  Sequence Link

  >RC211114 representing NM_003661
  Red=Cloning site Green=Tags(s)
  
  MEGAALLRVSVLCIWMSALFLGVGVRAEEAGARVQQNVPSGTDTGDPQSKPLGDWAAGTMDPESSIFIED
  AIKYFKEKVSTQNLLLLLTDNEAWNGFVAAAELPRNEADELRKALDNLARQMIMKDKNWHDKGQQYRNWF
  LKEFPRLKSKLEDNIRRLRALADGVQKVHKGTTIANVVSGSLSISSGILTLVGMGLAPFTEGGSLVLLEP
  GMELGITAALTGITSSTMDYGKKWWTQAQAHDLVIKSLDKLKEVREFLGENISNFLSLAGNTYQLTRGIG
  KDIRALRRARANLQSVPHASASRPRVTEPISAESGEQVERVNEPSILEMSRGVKLTDVAPVSFFLVLDVV
  YLVYESKHLHEGAKSETAEELKKVAQELEEKLNILNNNYKILQADQEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_003652
• RefSeq Size: 2856
• RefSeq ORF: 1194
• Synonyms: APO-L; APOL; APOL-I; FSGS4
• Locus ID: 8542
• UniProt ID: O14791, Q2KHQ6
• Cytogenetics: 22q12.3
• Summary: This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
• Protein Families: Secreted Protein, Transmembrane