HIST1H2BC (NM_003526) Human Mass Spec Standard

CAT#: PH317796

HIST1H2BC MS Standard C13 and N15-labeled recombinant protein (NP_003517)

Product Images

Coomassie blue staining of purified HIST1H2BC protein (Cat# TP317796). The protein was produced from HEK293T cells transfected with HIST1H2BC cDNA clone (Cat# RC217796) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC217796
• Predicted MW: 13.7 kDa
• Protein Sequence:
  Sequence Link

  >RC217796 representing NM_003526
  Red=Cloning site Green=Tags(s)
  
  MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDI
  FERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_003517
• RefSeq Size: 438
• RefSeq ORF: 378
• Synonyms: dJ221C16.3; H2B.1; H2B/l; H2BFL
• Locus ID: 8347
• UniProt ID: P62807, B2R4S9
• Cytogenetics: 6p22.2
• Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Systemic lupus erythematosus