AMD1 (NM_001634) Human Mass Spec Standard

CAT#: PH321424

AMD1 MS Standard C13 and N15-labeled recombinant protein (NP_001625)

Product Images

Coomassie blue staining of purified AMD1 protein (Cat# TP321424). The protein was produced from HEK293T cells transfected with AMD1 cDNA clone (Cat# RC221424) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC221424
• Predicted MW: 38.2 kDa
• Protein Sequence:
  Sequence Link

  >RC221424 representing NM_001634
  Red=Cloning site Green=Tags(s)
  
  MEAAHFFEGTEKLLEVWFSRQQPDANQGSGDLRTIPRSEWDILLKDVQCSIISVTKTDKQEAYVLSESSM
  FVSKRRFILKTCGTTLLLKALVPLLKLARDYSGFDSIQSFFYSRKNFMKPSHQGYPHRNFQEEIEFLNAI
  FPNGAAYCMGRMNSDCWYLYTLDFPESRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIR
  DLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSYDDLIRKVVEVFKPGKF
  VTTLFVNQSSKCRTVLASPQKIEGFKRLDCQSAMFNDYNFVFTSFAKKQQQQQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001625
• RefSeq Size: 3421
• RefSeq ORF: 1002
• Synonyms: ADOMETDC; AMD; SAMDC
• Locus ID: 262
• UniProt ID: P17707, B4DZ60, A0A088AWN0, Q6N0B2
• Cytogenetics: 6q21
• Summary: 'This gene encodes an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Multiple alternatively spliced transcript variants have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y. [provided by RefSeq, Dec 2013]'
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways