Oxytocin neurophysin 1 (OXT) (NM_000915) Human Mass Spec Standard

CAT#: PH324226

OXT MS Standard C13 and N15-labeled recombinant protein (NP_000906)

Product Images

Coomassie blue staining of purified OXT protein (Cat# TP324226). The protein was produced from HEK293T cells transfected with OXT cDNA clone (Cat# RC224226) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC224226
• Predicted MW: 12.72 kDa
• Protein Sequence:
  Sequence Link

  >RC224226 representing NM_000915
  Red=Cloning site Green=Tags(s)
  
  MAGPSLACCLLGLLALTSACYIQNCPLGGKRAAPDLDVRKCLPCGPGGKGRCFGPNICCAEELGCFVGTA
  EALRCQEENYLPSPCQSGQKACGSGGRCAVLGLCCSPDGCHADPACDAEATFSQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_000906
• RefSeq Size: 512
• RefSeq ORF: 375
• Synonyms: OT; OT-NPI; OXT-NPI
• Locus ID: 5020
• UniProt ID: P01178, X5D7M6
• Cytogenetics: 20p13
• Summary: 'This gene encodes a precursor protein that is processed to produce oxytocin and neurophysin I. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. [provided by RefSeq, Dec 2013]'
• Protein Families: Secreted Protein