Nkx2.6 (NKX2-6) (NM_001136271) Human Mass Spec Standard

CAT#: PH326770

NKX2 MS Standard C13 and N15-labeled recombinant protein (NP_001129743)

Product Images

Coomassie blue staining of purified NKX2-6 protein (Cat# TP326770). The protein was produced from HEK293T cells transfected with NKX2-6 cDNA clone (Cat# RC226770) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC226770
• Predicted MW: 23.1 kDa
• Protein Sequence:
  Sequence Link

  >RC226770 representing NM_001136271
  Red=Cloning site Green=Tags(s)
  
  MDAERMGEPQPGLNAASPLGGGTRVPERGVGNSGDSVRGGRSEQPKARQRRKPRVLFSQAQVLALERRFK
  QQRYLSAPEREHLASALQLTSTQVKIWFQNRRYKCKRQRQDKSLELAGHPLTPRRVAVPVLVRDGKPCLG
  PGPGAPAFPSPYSAAVSPYSCYGGYSGAPYGAGYGTCYAGAPSGPAPHTPLASAGFGHGGQNATPQGHLA
  ATLQGVRAW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001129743
• RefSeq ORF: 657
• Synonyms: CSX2; CTHM; NKX2F; NKX4-2
• Locus ID: 137814
• UniProt ID: A6NCS4
• Cytogenetics: 8p21.2
• Summary: This gene encodes a homeobox-containing protein that belongs to the NK-2 homeobox family. This protein is a vertebrate homolog of Drosophila homeobox-containing protein called 'tinman', which has been shown to be essential for development of the heart-like dorsal vessel. In conjunction with related gene, NKX2-5, this gene may play a role in both pharyngeal and cardiac embryonic development. Mutations in this gene are associated with persistent truncus arteriosus. [provided by RefSeq, Aug 2011]