SMAD6 (NM_001142861) Human Mass Spec Standard

CAT#: PH327610

SMAD6 MS Standard C13 and N15-labeled recombinant protein (NP_001136333)

Product Images

Coomassie blue staining of purified SMAD6 protein (Cat# TP327610). The protein was produced from HEK293T cells transfected with SMAD6 cDNA clone (Cat# RC227610) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC227610
• Predicted MW: 26.1 kDa
• Protein Sequence:
  Sequence Link

  >RC227610 representing NM_001142861
  Red=Cloning site Green=Tags(s)
  
  MSRMGKPIETQKSPPPPYSRLSPRDEYKPLDLSDSTLSYTETEATNSLITAPGEFSDASMSPDATKPSHW
  CSVAYWEHRTRVGRLYAVYDQAVSIFYDLPQGSGFCLGQLNLEQRSESVRRTRSKIGFGILLSKEPDGVW
  AYNRGEHPIFVNSPTLDAPGGRALVVRKVPPGYSIKVFDFERSGLQHAPEPDAADGPYDPNSVRISFAKG
  WGPCYSRQFITSCPCWLEILLNNPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001136333
• RefSeq ORF: 705
• Synonyms: AOVD2; HsT17432; MADH6; MADH7
• Locus ID: 4091
• Cytogenetics: 15q22.31
• Summary: 'The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]'
• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway