SARS-CoV-2 N Protein (NC_045512) Virus Over-expression Lysate

CAT#: LY490001

Transient overexpression lysate of SARS-CoV-2 nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2)

Product Images

Specifications

Product Data

• Species: Virus
• Expression cDNA Clone or AA Sequence: TrueORF Clone VC102555
• Tag: C-MYC/DDK
• Detection Antibodies: Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
• Accession Number: NC_045512
• Predicted MW: 45.6 kDa
• Components: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Storage: Ship with Dry ice. Store at -80°C after receiving vials. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Bioactivity: N/A
• Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.

Reference Data

• RefSeq: YP_009724397