PPIL1 (1-166, His-tag) Human Protein

CAT#: AR09117PU-N

PPIL1 (1-166, His-tag) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPDL KFTGAGILAM ANAGPDTNGS QFFVTLAPTQ WLDGKHTIFG RVCQGIGMVN RVGMVETNSQ DRPVDDVKII KAYPSGLEHH HHHH
• Tag: His-tag
• Predicted MW: 19.3 kDa
• Concentration: lot specific
• Purity: >95% by SDS PAGE
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl pH 8.0, 20% glycerol
• Bioactivity: Specific: > 300 nmoles/min/mg, defined as the amount of enzyme that cleaves
  1 umole of suc-AAFP-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin
• Preparation: Liquid purified protein
• Applications: Protocol: Activity Assay
  1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
  The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin.
  2. Add 10 ul of recombinant PPIL1 protein with 1 ug in assay buffer.
  3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
  4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
  5. Record the increase in A405 nm for 30 minutes at 25°C.
• Protein Description: Recombinant PPIL1, fused to His-tag at C-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
• Storage: Store (in aliquots) at -20°C or -70°C. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_057143
• Locus ID: 51645
• UniProt ID: Q9Y3C6, A0A024RCX8
• Cytogenetics: 6p21.2
• Synonyms: CGI-124; CYPL1; hCyPX; PPIase
• Summary: This gene is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved, ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. Based on similarity to other PPIases, this protein could accelerate the folding of proteins and might catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. [provided by RefSeq, Jul 2008]
• Protein Pathways: Spliceosome