NDRG1 (1-394, His-tag) Human Protein

CAT#: AR09269PU-N

NDRG1 (1-394, His-tag) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MSREMQDVDL AEVKPLVEKG ETITGLLQEF DVQEQDIETL HGSVHVTLCG TPKGNRPVIL TYHDIGMNHK TCYNPLFNYE DMQEITQHFA VCHVDAPGQQ DGAASFPAGY MYPSMDQLAE MLPGVLQQFG LKSIIGMGTG AGAYILTRFA LNNPEMVEGL VLINVNPCAE GWMDWAASKI SGWTQALPDM VVSHLFGKEE MQSNVEVVHT YRQHIVNDMN PGNLHLFINA YNSRRDLEIE RPMPGTHTVT LQCPALLVVG DSSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLDGT RSRSHTSEGT RSRSHTSEGT RSRSHTSEGA HLDITPNSGA AGNSAGPKSM EVSCLEHHHH HH
• Tag: His-tag
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 mM PMSF, 10% Glycerol
• Bioactivity: Biological: The ED50 for this effect is 0.5 - 1.5 ng/ml. Measured in a cell proliferation assay using MCF7 cell.
  Activity Assay
  1. Cell line: MCF7 (Human breast adenocarcinoma cell)
  2. Maintenance Condition: RPMI 1640 containing 10% FBS
  3. Assay medium: serum free RPMI 1640
  4. Cell density: 2 x 10e4 cells/well (96 well plate, final volume 100ul)
  5. Incubation time : 40 hrs (after sample treatment)
  6. Concentration range: 0.19ng/ml - 50ng/ml
  7. Detection method : BrdU assay
• Preparation: Liquid purified protein
• Protein Description: Recombinant human NDRG1, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001128714
• Locus ID: 10397
• UniProt ID: Q92597
• Cytogenetics: 8q24.22
• Synonyms: CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5
• Summary: This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]