GST-Tag (1-224, His-tag) Schistosoma Protein

CAT#: AR09284PU-N

GST-Tag (1-224, His-tag) schistosoma recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Schistosoma
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR
• Tag: His-tag
• Predicted MW: 28.3 kDa
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: PBS, pH 7.4, 10% glycerol
• Bioactivity: Biological: 2.8-3.3 units/mg, defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
  Activity Assay
  1. Prepare a 1 ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol.
  2. Equilibrate to 25°C and monitor at A340nm until the value is constant using a spectrophotometer.
  3. Add 50 ul of recombinant GST protein with various concentrations (1ug, 2ug, 5ug) in 950 ul reaction buffer.
  4. Mix by inversion and record the increase at A340nm for 5 minutes.
• Preparation: Liquid purified protein
• Protein Description: Recombinant GST, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -80°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.