UGDH (1-494, His-tag) Human Protein

CAT#: AR09548PU-N

UGDH (1-494, His-tag) human recombinant protein, 50 µg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV
• Tag: His-tag
• Predicted MW: 59.5 kDa
• Concentration: lot specific
• Purity: >95% by SDS – PAGE
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA
• Bioactivity: Specific: . One unit will oxidize 1.0 umol of UDP-glucose to UDP-glucuronic acid per minute at pH 8.0 at 25°C.
  Activity Assay
  1. Prepare a 1470 ul assay buffer. The final concentrations are 93mM Tris-Hcl (pH 8.0), 0.2mM UDPG, and 1mM beta-NAD.
  2. Add 20 ul of recombinant protein UGDH with 1 ug, 2 ug and 5 ug in assay buffer
  3. Mix by inversion and load 200 ul of reaction mix in to a plate well.
  4. Record the decrease in A340nm for 5 minutes at 25°C.
• Preparation: Liquid purified protein
• Protein Description: Recombinant human UGDH protein, fused to his-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001171629
• Locus ID: 7358
• UniProt ID: O60701
• Cytogenetics: 4p14
• Synonyms: GDH; UDP-GlcDH; UDPGDH; UGD
• Summary: 'The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]'
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Ascorbate and aldarate metabolism, Metabolic pathways, Pentose and glucuronate interconversions, Starch and sucrose metabolism