DHFR / DHFRP1 (1-187, His-tag) Mouse Protein
Other products for "Dhfr"
Specifications
Product Data | |
Species | Mouse |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD
|
Tag | His-tag |
Predicted MW | 23.8 kDa |
Concentration | lot specific |
Purity | >95% |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl |
Bioactivity | Specific: > 0.2 units/mg, in which one unit will convert 1.0 umole of 7,8– dihydrofloate and beta - NADPH to 5,6,7,8 - tetrahydrofloate and beta - NADP per min at pH 6.5 at 25°C Activity Assay: 1. Prepare a 1.55 ml assay buffer. The final concentrations are 50 mM potassium phosphate, 0.072 mM dihydrofolic acid, 0.1 mM beta-nicotinamide dinucleotide phosphate and 0.003 % (w/v) bovine serum albumin. 2. Add 50 ul of recombinant DHFR protein in various concentrations (2 ug, 5 ug) in assay buffer. 3. Mix by inversion and record the decrease at A340nm for 5 minutes. |
Preparation | Liquid purified protein |
Protein Description | Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
RefSeq | NP_034179 |
Locus ID | 13361 |
UniProt ID | P00375, Q544T5 |
Cytogenetics | 13 47.64 cM |
Synonyms | 8430436I03Rik; AA607882; AI662710; AW555094 |
Summary | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. [UniProtKB/Swiss-Prot Function] |
Documents
FAQs |
Resources
Recombinant Protein Resources |
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